HOME / Lb solid medium containing kanamycin

Lb solid medium containing kanamycin

Characterization and Evaluation of Lactic Acid Bacteria from

Antibiotic overuse has contributed to the emergence of multidrug-resistant (MDR) bacteria, posing a serious public health threat. Pets may act as reservoirs of MDR bacteria, with the potential to

Identification and recombinant expression of a novel

The recovered electroporated R. jostii RHA1 cells were directly plated on kanamycin containing (50 mg/L) LB agar plates and were incubated at 30°C. The R. jostii RHA1 transformed mutant

Characterization of the Nicotinamide Adenine Dinucleotide

The heat shock method was used to transfer the target gene fragment into E. coli DH5α, and strains were screened using LB medium containing the appropriate kanamycin concentration

Development of a DNA endonuclease I-SceI-based scarless

All E. coli strains were cultured in Luria–Bertani (LB) liquid medium or on solid medium with 1.5% agar at 37°C. Depending on the plasmid, 50 µg/mL kanamycin (Km) and 35 µg/mL

Ion channel structure and function of the MERS coronavirus

The transformed cells were cultured overnight in 30 ml of LB medium containing kanamycin (50 μg/ml) at 37°C with shaking at 220 rpm. The following day, the culture was transferred to 1.5

Transcription Factor IAA27 Positively Regulates P

The A. rhizogenes positive colonies were transferred to LB liquid medium containing 50 mg/L rifampicin and kanamycin by shaking at 28 °C for approximately 10 h. The bacterial liquid was centrifuged for 8 min at 7000× g

Cloning and expression of the dimethylsulfoniopropionate

This recombinant plasmid was transformed into Escherichia coli BL21 (DE3) cells, which were then induced with isopropyl β -D-1-thiogalactopyranoside (IPTG) at 16°C for a duration of 10

Transcriptome-assisted sugar transporter screening for

The identified positive colonies were inoculated into LB liquid medium containing the corresponding antibiotics and pre-cultured at 37 °C in a shaking incubator at 200 rpm for 8–12

Development of an efficient heterologous protein expression

For plasmid construction and selection, Escherichia coli Tran1-T1 was cultured at 37 °C in Luria–Bertani (LB) medium supplemented with kanamycin (50 mg/L) or ampicillin (100 mg/L)

Reversion of a RND transporter pseudogene reveals latent

Mutations identified in six independent spontaneous mutants resistant to cilnidipine, isolated after growth on solid medium containing 25 µM cilnidipine. The locations of the bepE frameshift

Undescribed indanomycin derivatives from ΔidmH mutant of

Escherichia coli stains were cultured in LB liquid or solid medium containing the appropriate antibiotics unless otherwise specified. The following antibiotics were used for the selective

Engineering and laboratory evolution of lactose-utilizing

Thereafter, the engineered strain KT2440Gal was adapted on minimal medium containing lactose as an only carbon source. Three lactose-utilizing mutants were further characterized for their

Lb solid medium containing kanamycin [PDF]

Solar Pro.